Figure 3.

GPR18 does not play a role in the DSS model of colitis. Colitis was induced in Gpr18^-/- and control littermates (Gpr18^+/- ) by two cycles of 2.5% DSS for 5 days followed by two days of water and disease progression was monitored. (A) Mice were weighed daily for 10 days and bodyweight change as % starting weight was determined. Data was combined from three independent experiments. (B) Colon lengths at end point. (C) H&E staining of representative distal colon and cecum sections from at 40x (scale bar 200 μm) magnification. (D) Blinded histology scores from distal colon and cecum combined from 3 independent experiments. The representative images were taken from mice represented by the orange colored data points. (E) Percentage of total ILC isolated from the colon lamina propria from two independent DSS colitis experiments. Total ILC were gated as Thy1.2⁺CD45⁺LIN^-CD3^-, Lineage markers were CD19, B220, CD11c and Gr1. (F) Percentage of ILC2 and IlC3 isolated from the colon lamina propria from two independent DSS experiments. ILC2 were gated as CD45⁺LIN^-CD3^-Thy1.2⁺Eomes^-GATA3⁺ and ILC3 were gated as CD45⁺ LIN^-CD3^-Thy1.2⁺Eomes^-RORγt⁺. Error bars represent S.E.M. Two-way ANOVA (Tukey’s test) (A) and nonparametric T-test (Mann-Whitney test) (B, D–F). ns, not significant.