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. 2022 Oct 28;13:1034648. doi: 10.3389/fimmu.2022.1034648

Figure 4.

Figure 4

GPR18 expression by T cells is not required for colitis in the transfer model. Colitis was induced by adoptive transfer of GPR18 deficient (Gpr18-/- ) vs. littermate control (Gpr18+/- ) naive CD4+ T cells into Rag1-/- recipients. (A) Weight change was monitored as % starting body weight on the indicated days. A representative weight graph from one of 3 independent experiments is shown. The weight of dead mice was carried forward, indicated with a + sign in magenta for one mouse for Gpr18+/- T cells. (B) H&E staining of representative colon sections (middle part of whole colon) at 40x (scale bar 100 μm) magnification. (C) Blinded histology scores from the middle part of the whole colon of 3 independent experiments combined. Representative images were taken from mice represented by the orange colored data points. Error bars represent S.E.M. Two-way ANOVA (Tukey’s test) (A) and nonparametric T-test (Mann-Whitney test) (C). ns, not significant.