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. 2022 Jul 28;6(11):1272–1283. doi: 10.1038/s41551-022-00911-4

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© The Author(s) 2022, corrected publication 2022

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Extended Data Fig. 1 — Quantification of AAV genomes from tissue encoding SaABE8e dual AAV (SaABE8e with intact editor on one genome and sgRNA and EGFP on a second genome) or single AAV (SaABE8e and guide RNA all-in-one), both installing Pcsk9 W8R packaged in AAV9. Editors were packaged in AAV9 and administered by retro-orbital injection at 6–8 weeks of age at the dose indicated in the legend (dual AAVs were delivered at the dose indicated in the legend of editor AAV and sgRNA AAV, while single AAVs were delivered at the total dose indicated). Tissues were harvested 3 weeks post injection then editor AAV genomes were quantified by ddPCR using SaCas9 primers and probe, normalized to Gapdh. Dots represent individual mice (n = 1–3) and error bars show s.e.m.