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. 2022 Jul 28;6(11):1272–1283. doi: 10.1038/s41551-022-00911-4

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© The Author(s) 2022, corrected publication 2022

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Extended Data Fig. 3 — a, Liver tissue from single-AAV ABE treated mice. The top three predicted off-target sites for SaKKH-ABE8e targeting Pcsk9 exon 1 splice donor were sequenced from liver tissue. b, The editing observed at OT2 is dose-dependent. c, On- and off-target sites were sequenced after plasmid transfection of N2A cells with sgRNA targeting Pcsk9 exon 1 donor site and full-length or intein-split SpABE8e. Full-length and intein-split SpABE8e did not significantly differ in efficiency at on- or off-target edits by multiple unpaired t tests with Holm-Sidak method for correction for multiple comparisons. OT, off-target; NT, non-targeting dose-matched dual AAV8 ABE7.10 targeting Dnmt1, an unrelated gene.