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. 2022 Nov 11;5:1178. doi: 10.1038/s42003-022-04077-2

Fig. 6. TTX decreased Vm fluctuations in MDA-MB-231 cells.

Fig. 6

ai, ii Example Vm time courses from the 8 most active cells in acquisitions before (ai) and with (aii) 10 μM TTX. bi, bii Log-scaled 2D histograms of detected events before (bi) and with (bii) 10 μM TTX. TTX eradicates virtually all large, hyperpolarizing events (−VEs, dashed orange outline). c Log-scaled histograms of the −VE event rate per cell for pre- and post-application of 10 μM TTX. The reduction in the mean from 9.5 × 10−5 events/cell/s to 1.97 × 10−5 events/cell/s (~4× decrease) is significant with p < 10−6. d The effect of TTX is dose-dependent: 1 μM TTX reduced the mean event rate by a lesser factor of ~2× from 1.04 × 10−4 events/cell/s to 4.99 × 10−5 events/cell/s (p = 0.019). e The effect of TTX is reversible. The effects of TTX can be reversed by washing out the toxin, significantly increasing the −VE rate.