Table 3.
Area at risk, baseline temperature, regional myocardial blood flow, heart rate, and left ventricular pressure in V/V and I/I Ossabaw minipigs undergoing ischaemia/reperfusion without and with prior ischaemic preconditioning
| Ischaemia/reperfusion | Ischaemic preconditioning + Ischaemia/reperfusion |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| V/V (n = 15) |
I/I (n = 16) |
V/V (n = 16) |
I/I (n = 15) |
||||||||||||
| AAR [%LV] | 24 ± 6 | 20 ± 5 | 25 ± 7 | 24 ± 5 | |||||||||||
| TEMP [°C] | 38.1 ± 0.5 | 38.1 ± 0.7 | 38.2 ± 0.5 | 38.1 ± 0.8 | |||||||||||
|
RMBF [ml/min/g] |
Time p < 0.0001 | ||||||||||||||
| BL | 0.96 ± 0.22 | 1.01 ± 0.34 | 0.96 ± 0.18 | 1.17 ± 0.49 | |||||||||||
| after IPC | 1.12 ± 0.36 | 1.45 ± 0.43 | |||||||||||||
| I5 | 0.04 ± 0.02 | 0.04 ± 0.03 | 0.05 ± 0.03 | 0.07 ± 0.04 | |||||||||||
| I55 | 0.03 ± 0.03 | 0.03 ± 0.02 | 0.03 ± 0.02 | 0.03 ± 0.02 | |||||||||||
| R10 | 1.47 ± 0.66 | 1.15 ± 0.67 | 1.05 ± 0.62 | 1.12 ± 0.50 | |||||||||||
| R180 | 0.67 ± 0.30 | 0.67 ± 0.29 | 0.63 ± 0.22 | 0.83 ± 0.59 | |||||||||||
|
HR [1/min] |
Time p < 0.0001 | Main effect genotype p = 0.0035 | |||||||||||||
| BL | 103 ± 6 | 96 ± 7 | 99 ± 3 | 99 ± 8 | |||||||||||
| after IPC | 103 ± 4 | 99 ± 4 | |||||||||||||
| I5 | 102 ± 4 | 98 ± 5 | 103 ± 5 | 100 ± 4 | |||||||||||
| I55 | 102 ± 8 | 96 ± 9 | 103 ± 11 | 98 ± 7 | |||||||||||
| R10 | 101 ± 8 | 97 ± 9 | 102 ± 11 | 101 ± 5 | |||||||||||
| R180 | 106 ± 8 | 102 ± 11 | 108 ± 15 | 100 ± 10 | |||||||||||
|
LVP [mmHg] |
Time p < 0.00001 | Main effect protocol p = 0.0391 | |||||||||||||
| BL | 86 ± 8 | 93 ± 8 | 85 ± 6 | 88 ± 7 | |||||||||||
| after IPC | 82 ± 8 | 84 ± 6 | |||||||||||||
| I5 | 79 ± 11 | 82 ± 12 | 77 ± 10 | 79 ± 5 | |||||||||||
| I55 | 77 ± 10 | 82 ± 15 | 72 ± 13 | 73 ± 10 | |||||||||||
| R10 | 75 ± 7 | 80 ± 13 | 71 ± 13 | 76 ± 7 | |||||||||||
| R180 | 75 ± 8 | 80 ± 11 | 68 ± 13 | 74 ± 6 | |||||||||||
Area at risk (AAR), temperature before sustained coronary occlusion (TEMP), regional myocardial blood flow (RMBF), heart rate (HR), and left ventricular pressure (LVP) in Ossabaw minipigs with a V/V genotype (pigs with homozygous valine in the γ-subunit of the adenosine monophosphate-activated protein kinase) and I/I genotype (pigs with homozygous isoleucine in the γ-subunit of the adenosine monophosphate-activated protein kinase) undergoing myocardial ischaemia/reperfusion without or with prior ischaemic preconditioning (IPC). Two-way (genotype, protocol) analysis of variance for AAR and temperature; three-way (genotype, protocol, time) analysis of variance for RMBF, HR, and LVP, each with least square means tests
BL baseline; I5 5 min ischaemia; I55 55 min ischaemia; R10 10 min reperfusion; R180 180 min reperfusion