Table 4.
Area at risk, baseline temperature, regional myocardial blood flow, heart rate, and left ventricular pressure in female and castrated male Ossabaw minipigs undergoing ischaemia/reperfusion without and with prior ischaemic preconditioning
| Ischaemia/reperfusion | Ischaemic preconditioning + ischaemia/reperfusion |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Female (n = 15) |
Castrated male (n = 16) | Female (n = 15) |
Castrated male (n = 16) | ||||||||||||
| AAR [%LV] | 24 ± 7 | 20 ± 4 | 24 ± 5 | 24 ± 13 | |||||||||||
| TEMP [°C] | 38.2 ± 0.8 | 38.0 ± 0.4 | 38.5 ± 0.6 | 37.9 ± 0.6 | |||||||||||
|
RMBF [ml/min/g] |
Time p < 0.0001 | ||||||||||||||
| BL | 1.04 ± 0.32 | 0.94 ± 0.25 | 1.17 ± 0.46 | 0.98 ± 0.39 | |||||||||||
| after IPC | 1.20 ± 0.46 | 1.36 ± 0.03 | |||||||||||||
| I5 | 0.04 ± 0.03 | 0.04 ± 0.03 | 0.05 ± 0.04 | 0.06 ± 0.02 | p < 0.001 vs. BL, R10, R180 | ||||||||||
| I55 | 0.03 ± 0.02 | 0.03 ± 0.02 | 0.03 ± 0.03 | 0.03 ± 0.52 | p < 0.001 vs. BL, R10, R180 | ||||||||||
| R10 | 1.45 ± 0.66 | 1.18 ± 0.68 | 1.01 ± 0.58 | 1.16 ± 0.23 | |||||||||||
| R180 | 0.77 ± 0.28 | 0.56 ± 0.26 | 0.74 ± 0.58 | 0.69 ± 0.23 | |||||||||||
|
HR [1/min] |
Time p < 0.0001 | ||||||||||||||
| BL | 101 ± 7 | 98 ± 7 | 100 ± 4 | 98 ± 4 | |||||||||||
| after IPC | 103 ± 5 | 100 ± 2 | |||||||||||||
| I5 | 101 ± 4 | 98 ± 5 | 103 ± 6 | 100 ± 7 | |||||||||||
| I55 | 99 ± 11 | 99 ± 7 | 100 ± 12 | 101 ± 7 | |||||||||||
| R10 | 99 ± 7 | 99 ± 10 | 100 ± 10 | 102 ± 13 | |||||||||||
| R180 | 105 ± 9 | 103 ± 11 | 104 ± 10 | 104 ± 23 | |||||||||||
|
LVP [mmHg] |
Time p < 0.00001 | Main effect protocol p = 0.0397 | |||||||||||||
| BL | 89 ± 9 | 90 ± 9 | 87 ± 7 | 86 ± 7 | |||||||||||
| after IPC | 83 ± 7 | 83 ± 10 | |||||||||||||
| I5 | 77 ± 12 | 84 ± 10 | 77 ± 5 | 79 ± 12 | |||||||||||
| I55 | 75 ± 13 | 84 ± 12 | 70 ± 11 | 75 ± 10 | |||||||||||
| R10 | 76 ± 10 | 79 ± 11 | 70 ± 11 | 77 ± 9 | |||||||||||
| R180 | 76 ± 8 | 80 ± 11 | 68 ± 12 | 74 ± 10 | |||||||||||
Area at risk (AAR), temperature before sustained coronary occlusion (TEMP), regional myocardial blood flow (RMBF), heart rate (HR), and left ventricular pressure (LVP) in female and castrated male Ossabaw minipigs undergoing myocardial ischaemia/reperfusion without or with prior ischaemic preconditioning (IPC). Two-way (sex, protocol) analysis of variance for AAR and temperature; three-way (sex, protocol, time) analysis of variance for RMBF, HR, and LVP, each with least square means tests
BL baseline; I5 5 min ischaemia; I55 55 min ischaemia; R10 10 min reperfusion; R180 180 min reperfusion