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. 1999 Apr;67(4):1743–1749. doi: 10.1128/iai.67.4.1743-1749.1999

FIG. 4.

FIG. 4

Strains N40 and HB19 clone 1 demonstrate differences in their preferences for dermatan sulfate or heparin. (A) 293 cells were mock treated or were treated with the indicated lyase(s) prior to the addition of radiolabeled bacteria, and the percent bound bacteria was determined (see Materials and Methods). Hep., heparinase digestion; Hpt., heparitinase digestion; Chon. ABC, chondroitinase ABC digestion. Each bar represents the average ± the SD of four determinations. Significant (P < 0.05) differences in binding to mock- versus chondroitinase-treated monolayers were determined by two-tailed t-test analysis and are indicated by single asterisks. Double asterisks indicate significant differences in binding to chondroitinase versus combination lyase digestions. (B) Attachment of strain N40 and HB19 clone 1 was determined in the presence of various concentrations of heparin or dermatan sulfate (see Materials and Methods). Binding is expressed relative to the binding in the absence of inhibitor. In the experiment shown, the binding of N40 in the absence of inhibitor was 28.1% of the inoculum, and binding of HB19 clone 1 was 16.4%.