TABLE 4.
Different GAG inhibition profiles expressed by two B. burgdorferi strains
| Inhibitor | IC50 (μg/ml)a for strain:
|
|
|---|---|---|
| N40 | HB19 clone 1 | |
| Heparin | 1.3 | >500 |
| Dermatan sulfate | 60 | 18 |
| Chondroitin-6-sulfate | >500 | >500 |
| Chondroitin-4-sulfate | >500 | >500 |
a
Attachment of B. burgdorferi N40 and HB19 to 293 cells was determined in the presence of various concentrations of GAG (see Materials and Methods). IC50 indicates the estimated concentration of GAG at which bacterial binding is 50% of the level of binding in the absence of inhibitor. The percentages of the spirochetes bound in the absence of inhibitors were as follows: N40, 28.3 ± 2.8%; HB19 clone 1, 18.1 ± 2.7%.