Table 5.
Short term effect of high salt (4% in chow) diet followed by Amoxicillin treatment on the mean relative abundance of major bacterial groups at phylum level in mice.
| Phylum | Relative abundance (%) | Significance (Adjusted p-value) | |||||
|---|---|---|---|---|---|---|---|
| CD | CD + A | HSD | HSD + A | CD versus CD + A | CD versus HSD | HSD versus HSD + A | |
| Firmicutes | 80.30 ± 0.397 | 1.80 ± 0.132 | 47.40 ± 0.499 | 9.80 ± 0.297 | < 0.0001 | 0.0028 | 0.0009 |
| Bacteroidetes | 5.10 ± 0.219 | 56.30 ± 0.496 | 2.30 ± 0.149 | 5.90 ± 0.235 | < 0.0001 | < 0.0001 | < 0.0001 |
| Proteobacteria | 7.50 ± 0.264 | 34.00 ± 0.473 | 16.20 ± 0.368 | 65.3 ± 0.467 | < 0.0001 | < 0.0001 | < 0.0001 |
| Actinobacteria | 3.90 ± 0.193 | 3.60 ± 0.186 | 9.30 ± 0.290 | 5.20 ± 0.022 | NS | < 0.0001 | < 0.0001 |
| Cyanobacteria | 0.50 ± 0.070 | 0.90 ± 0.094 | 0.60 ± 0.077 | 0.60 ± 0.077 | < 0.0001 | 0.0159 | NS |
| Acidobacteria | 0.40 ± 0.063 | 0.50 ± 0.070 | 1.00 ± 0.099 | 1.10 ± 0.104 | 0.0264 | < 0.0001 | < 0.0001 |
| Verrucomicrobia | 0.20 ± 0.044 | 0.40 ± 0.063 | 6.70 ± 0.250 | 8.10 ± 0.272 | < 0.0001 | < 0.0001 | < 0.0001 |
| Chlorobi | 0.10 ± 0.031 | 0.20 ± 0.044 | 0.10 ± 0.031 | 0.10 ± 0.031 | 0.0011 | NS | NS |
| Chloroflexi | 0.20 ± 0.044 | 0.30 ± 0.054 | 0.50 ± 0.070 | 0.60 ± 0.077 | 0.0056 | < 0.0001 | NS |
| TM7 | 0.10 ± 0.031 | 0.10 ± 0.031 | 11.60 ± 0.320 | 0.20 ± 0.044 | NS | < 0.0001 | < 0.0001 |
| Gemmatimonadetes | 0.20 ± 0.044 | 0.20 ± 0.044 | 0.50 ± 0.070 | 0.50 ± 0.070 | NS | < 0.0001 | NS |
| Fusobacteria | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.30 ± 0.054 | 0.40 ± 0.063 | NS | < 0.0001 | 0.0076 |
| Nitrospirae | 0.10 ± 0.031 | 0.10 ± 0.031 | 0.20 ± 0.044 | 0.30 ± 0.054 | NS | 0.0040 | 0.0040 |
| Planctomycetes | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.10 ± 0.031 | 0.00 ± 0.000 | NS | < 0.0001 | < 0.0001 |
| Tenericutes | 0.00 ± 0.000 | 0.00 ± 0.000 | 0.50 ± 0.070 | 0.00 ± 0.000 | NS | < 0.0001 | < 0.0001 |
| F/B ratio | 15.74 ± 0.363 | 0.05 ± 0.022 | 20.6 ± 0.404 | 1.66 ± 0.125 | < 0.0001 | < 0.0001 | < 0.0001 |
Values are expressed as the means ± SD. Data were analyzed by one-way ANOVA, followed by Bonferroni test, n = 6, p ≤ 0.05, NS = Not significant, CD: Standard—chow diet for 3 weeks; CD + A: Standard—chow diet for 3 weeks and subjected to Amoxicillin treatment in 3rd week; HSD: High-salt diet for 3 weeks; HSD + A: High-salt diet for 3 weeks and subjected to Amoxicillin treatment in 3rd week.