Skip to main content
. 2022 Nov 11;13:6871. doi: 10.1038/s41467-022-34639-1

Fig. 1. Overall structure of human mini-SNAPc complexed with U6-1 PSE.

Fig. 1

a Schematic diagram of two types of human snRNA promoters to recruit Pol II or Pol III. b Five-subunit hSNAPc composition with detailed domain organization. SNAP190(1-505) is shown in green, SNAP50 in cyan, SNAP43(1-268) in magenta, and SNAP19 in pink. The domain–domain interactions are indicated using dash lines. The subunit or fragments not constructed for mSNAPc#2, including SNAP45, SNAP190(506-1469), and SNAP43(269-368), are shown in gray. The rod module containing SNAP19, SNAP190(1-143), and SNAP43(148-268) are indicated as dash boxes with asterisks. Mini-SNAPc is highlighted by a red frame. c Electrophoretic mobility shift assay (EMSA) of mSNAPc#2 with 25 bp human U6-1 PSE and non-PSE sequences. Increasing amounts of proteins (0, 200, 400, 600, 1200 nM) were incubated with 50 nM fluorescently labeled DNA probes. This experiment was repeated independently three times with similar results. d Cryo-EM structure of mini-SNAPc in complex with hU6-1 PSE. The 35 bp DNA sequence is numbered at the exact position of hU6-1 promoter, with 24 bp built into structure highlighted in orange and 18 bp PSE boxed in brown. The overall structure of this complex is shown in cartoon and density map. Color coding follows the same color scheme from Fig.1b (same as below unless otherwise specified). NTS non-template strand, TS template strand.