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. 2022 Nov 11;13:6871. doi: 10.1038/s41467-022-34639-1

Fig. 2. Mini-SNAPc assembles in a “wrap-around” mode.

Fig. 2

a Domain organizations of SNAP190(144-505). Four and a half MYB repeats (Rh, Ra, Rb, Rc, and Rd) are shown in dark green. Four regions of SNAP190 potentially interacting with SNAP50 are indicated. b The experimental structures of SNAP50 and SNAP43(4-147). The lasso domain, the β-barrel domain and the Wedge domain are shown in blue, cyan, and light blue, respectively. Two zinc atoms are labeled as gray dots. The NTD of SNAP43 is embedded into an interface formed by the lasso domain of SNAP50. c, d Domain–domain interaction analysis within mini-SNAPc using anti-FLAG affinity chromatograph. Western blots showed that Region I is the main segment of SNAP190 interacting with SNAP50, and SNAP50 relies on the Lasso domain to grab the NTD of SNAP43. Detailed in Method Section. This experiment was repeated independently three times with similar results.