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. 2022 Nov 11;13:6828. doi: 10.1038/s41467-022-34665-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — ELISA of Cxcl5, Cxcl9 and Cxcl10 in lysates of RM-1 tumors grown with control (Ctrl) and Foxf2-expressing mouse stromal cells (a) and supernatant of in vitro cultured control and Foxf2-expressing mouse prostate stromal cells (mPrSC) (b). Data represent means ± s.d. of protein expression. In Fig. 4, N = 9 per group for Cxcl5, N = 7 per group for Cxcl9 and Cxcl10. In Fig. 4b, N = 3 per group. Two-sided unpaired t-test. c qRT-PCR of Cxcl5/9/10 in FACS-isolated stromal, RM-1, and myeloid cells from RM-1 tumors grown with mouse prostate stromal cells. Data represent means ± s.d. of gene expression from 4 tumors per group. d, e qRT-PCR of Foxf2 and Cxcl5/9/10 in FACS-isolated stromal cells from RM-1 tumors grown with control and Foxf2-expressing stromal cells and from prostates of 9-mth-old control (Col1a2-TRAMP) and Col1a2-Foxf2-TRAMP mice. Dot plots show means ± s.d. of gene expression from 4 mice per group. f Image of RM-1 tumors grown with control and Foxf2-expressing stromal cells with scrambled shRNA or shRNAs against Cxcl5. Dot plot shows means ± s.d. of tumor weight. N = 8 per group. Two-way ANOVA with Tukey’s multiple comparison test. Bar = 1 cm. g Dot plots show means ± s.d. of percentages of immune cell lineages determined by flow cytometry. N = 7 per group except that N = 6 for analysis of macrophages in shCxcl5-2 group. Two-way ANOVA with Tukey’s multiple comparison test. h Image of RM-1 tumors grown with control and Foxf2-expressing stromal cells with scrambled shRNA or shRNAs against Cxcl9 and Cxcl10. Dot plot shows means ± s.d. of tumor weight. N = 8 per group. Two-way ANOVA with Tukey’s multiple comparison test. Bar = 1 cm. Source data are provided as a Source Data file.