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. 2022 Nov 11;5:1230. doi: 10.1038/s42003-022-04156-4

Fig. 3. Hypertrophy is characterized by a decrease in glycolysis and glycolytic capacity.

Fig. 3

a Schematic of expected extracellular acidification rate (ECAR) readings for a Glycolysis Stress Test in the Seahorse XFe96 metabolic analyzer, including injection points for glucose (G), oligomycin (O), and 2-deoxyglucose (2DG). b Metabolic analysis (ECAR) of WT epiphyseal chondrocytes under proliferative and hypertrophic conditions. c Metabolic analysis of WT and Igf2 null epiphyseal chondrocytes under proliferative and hypertrophic conditions. Note that the WT lines in (c) are the same as in (b). d Glycolysis, glycolytic capacity, and non-glycolytic acidification as calculated from ECAR measurements. Data are presented as individual measurements with a line indicating the mean (b, c) or individual measurements with mean + SEM (d), representative of n = 4 experiments with 3–6 wells per group. Statistical significance was calculated using a two-way ANOVA with Tukey’s test for multiple comparisons (d). p-values indicated as ns (not significant), *p < 0.05, **p < 0.01, ***p < 0.001.