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. 2022 Nov 11;12:19294. doi: 10.1038/s41598-022-23841-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of intravenous injection of SET-M33 at 0 (vehicle), 5, 9 and 15 mg/kg on respiratory rate (breaths/min ± se), tidal volume (mL ± se) and minute volume (mL/min ± se) in rats. Baclofen at 15 mg/day was used as positive control. se standard error of mean. Symbols (triangle, circle, rhombus or square) indicate a group mean respiratory parameter. The bar represents the standard error. The graphs were obtained using GraphPad Prism for Windows version 5.03, GraphPad Software, San Diego, California USA, www.graphpad.com. The groups treated with SET-M33 peptide were compared to vehicle using Williams’ test. The comparison between positive control and vehicle were made using two-tailed t test based on the error mean square from the analysis of covariance.