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. 2022 Nov 11;13:6849. doi: 10.1038/s41467-022-34490-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Fission activity in cellulo where BIN1-eGFP (green) and WT-DNM2 (magenta), M-DNM2 or Ub-DNM2, were co-expressed in COS-1 cells at incremental DNM2 concentration. Scale bar = 20 µm. b Fission activity in cellulo done as (a) co-expressing BIN1-eGFP (green) and the CNM mutant S619L-DNM2 (magenta). Scale bar = 20 µm. In both figures (a, b), percentage of each cell phenotype (tubular, intermediate or vesicular pattern) is shown at the right (average of 3 independent experiments). A representative image of the experiment at equal ratio of DNM2 and BIN1 is shown at the left. Data are represented as mean values ± SEM of a minimum of 25 cells/condition examined over 3 independent experiments. Source data are provided as a Source Data file.