TABLE 1.

Bacterial strains and plasmids

Strain or plasmid	Relevant characteristic(s)a	Source or reference
Strains
E. coli
CC118	PhoA−	21
XL2 blue		Stratagene
S. suis
10	Virulent serotype 2 strain	40
10cpsB	Isogenic cpsB mutant of strain 10	This work
10cpsEF	Isogenic cpsEF mutant of strain 10	This work
Plasmids
pKUN19	Replication functions pUC, Ampr	19
pGEM7Zf(+)	Replication functions pUC, Ampr	Promega Corp.
pIC19R	Replication functions pUC, Ampr	22
pIC20R	Replication functions pUC, Ampr	22
pIC-spc	pIC19R containing Spcr gene of pDL282	Lab collection
pDL282	Replication functions of pBR322 and pVT736-1, Ampr, Spcr	31
pPHOS2	pIC-spc containing the truncated phoA gene of pPHO7 as a PstI-BamHI fragment	This work
pPHO7	Contains truncated phoA gene	12
pPHOS7	pPHOS2 containing chromosomal S. suis DNA	This work
pCPS6	pKUN19 containing 6-kb HindIII fragment of cps operon	This work (Fig. 1)
pCPS7	pKUN19 containing 3.5-kb EcoRI-HindIII fragment of cps operon	This work (Fig. 1)
pCPS11	pCPS7 in which 0.4-kb PstI-BamHI fragment of cpsB gene is replaced by Spcr gene of pIC-spc	This work (Fig. 1)
pCPS17	pKUN19 containing 3.1-kb KpnI fragment of cps operon	This work (Fig. 1)
pCPS18	pKUN19 containing 1.8-kb SnaBI fragment of cps operon	This work (Fig. 1)
pCPS20	pKUN19 containing 3.3-kb XbaI-HindIII fragment of cps operon	This work (Fig. 1)
pCPS23	pGEM7Zf(+) containing 1.5-kb MluI fragment of cps operon	This work (Fig. 1)
pCPS25	pIC20R containing 2.5-kb KpnI-SalI fragment of pCPS17	This work (Fig. 1)
pCPS26	pKUN19 containing 3.0-kb HindIII fragment of cps operon	This work (Fig. 1)
pCPS27	pCPS25 containing 2.3-kb XbaI (blunt)-ClaI fragment of pCPS20	This work (Fig. 1)
pCPS28	pCPS27 containing the 1.2-kb PstI-XhoI Spcr gene of pIC-spc	This work (Fig. 1)

^aAmp^r, ampicillin resistant; Spc^r, spectinomycin resistant; and cps, capsular polysaccharide.