Escape of wild-type or mutant L. monocytogenes cells from the primary vacuole. The percentages of bacteria staining with phalloidin at 90 min are given for cells infected with wild-type L. monocytogenes (A), pretreated with SK&F 96365 (25 μM) and infected with wild-type L. monocytogenes (B), pretreated with thapsigargin (1 μM) and infected with wild-type L. monocytogenes (C), infected with the PI-PLC mutant (D), infected with the broad-range PLC mutant (E), and infected with the double-PLC mutant (F). Results are expressed as the mean ± standard error for three to six cell preparations. ∗, P < 0.05 for untreated cells infected with wild-type L. monocytogenes compared to wild-type-infected cells pretreated with SK&F 96365 (25 μM) or thapsigargin (1 μM) or for untreated J774 cells infected with wild-type L. monocytogenes compared to cells infected with the PI-PLC or double-PLC mutant.