Table 2.
Summary of the ADP-ribose detection reagent properties.
| PAR/MAR detection | Western blot | ADPr RNA | IF | Caution | |
|---|---|---|---|---|---|
| I Anti-MAR reagent | Suitable for MARylation | Good | Not suitable | Suitable with all fixatives | May depend on the backbone |
| II eAf1521-Fc | No distinction MAR/PARylation | Good | Suitable | Suitable with all fixatives | Retains catalytic activity |
| III mPARP14-macro2/3-Fc | Suitable for MARylation | Weak signal | Suitable | Suitable with all fixatives | May depend on the backbone |
| IV Anti-MAR antibody | No distinction MAR/PARylation | Good | Suitable | Yes, when fixed with MeOH or glyoxal | Cross-reacts with AMPylation and NADH |
| V Anti-PAR/MAR (E6F6A) | No distinction MAR/PARylation | Good | Suitable | Yes, when fixed with MeOH or glyoxal | Cross-reacts with NADH |
| VI Pan-ADPr | No distinction MAR/PARylation | Weak signal | Not suitable | Suitable with all fixatives | — |
| VII Anti-PAR antibody | Suitable for PARylation | Suitable for PARylation but high background | Not suitable | n.d. | Only PARylation |
| Anti-ADPr HCA354 | Suitable for MARylation | Good | Suitable | n.d. | Cross-reacts with AMPylation and NADH |
| Anti-ADPr HCA355 | Suitable for MARylation | Good | Not suitable | n.d. | — |