Table 4.

Conditions for recombinant protein expression.

Protein	Plasmid	Bacterial strain	OD600 at induction	IPTG [mM]	Time	Temp.	Source
MACROD1	pNH-TrxT-MACROD1_77-325	BL21-CodonPlus (DE3)-RIL	0.6	0.4	16 h	22°C	Chen et al (2011)
MACROD2	pDEST17-MACROD2	BL21-CodonPlus (DE3)-RIL	0.6	0.4	16 h	22°C	Rosenthal et al (2013)
TARG1	pDEST17-TARG1	BL21-CodonPlus (DE3)-RIL	0.6	0.4	16 h	22°C	Butepage et al (2018b)
PARGcat	pNH-TrxT_hPARG_cat_448-976	Rosetta (DE3)pLysS	0.6	0.4	16 h	18°C	Wazir et al (2021)
ARH1	pNIC-Bsa4_hARH1	BL21-CodonPlus (DE3)-RIL	0.6	1	16 h	18°C	Wazir et al (2021)
ARH3	pDEST17-ARH3	BL21-CodonPlus (DE3)-RIL	0.6	1	6 h	16°C	Kleine et al (2008)
PARP10cat	pGST-PARP10_818-1025	BL21-CodonPlus (DE3)-RIL	0.6	1	16 h	18°C	Kleine et al (2008)
mART2.2	pASK60-ART2.2-FlagHis6x	Lemo21(DE3) E. coli	0.6	1	16 h	16°C	Mueller-Dieckmann et al (2002)
Pertussis toxin_cat	pET15b-rPtxS1	BL21-CodonPlus (DE3)-RIL	0.6	0.4	16 h	18°C	Ashok et al (2020)
C3 botulinum	pGEX-C3-Cbot	Rosetta (DE3)pLysS	0.6	0.4	16 h	18°C	Pautsch et al (2005)
mPARP14-macro2/3-Fc	pET28-PARP14-macro2/3-Fc	Rosetta (DE3)pLysS	0.6	0.4	16 h	18°C	This study
mPARP14-macro2/3-GE-Fc	pET28-PARP14-macro2/3-Fc-GE	Rosetta (DE3)pLysS	0.6	0.4	16 h	18°C	This study