Table 6.
# | Reagent | Source | Catalogue number | Dilution used for Western blot | Dilution used for microscopy | Isotype | Reusable? | Ref |
---|---|---|---|---|---|---|---|---|
I | Anti-MAR | Millipore | MABE1076 | 1:1,000 | 1:500 | Rabbit IgG | No | Gibson et al (2017) |
II | eAf1521-Fc | Michael Hottiger | n/a | 1:1,000 | 1:50 | Mouse IgG | Yesa | Nowak et al (2020) |
III | mPARP14-macro2/3-Fc | Bernhard Lüscher | n/a | 1:1,000 | 1:50 | Mouse IgG | No | — |
IIIm | mPARP14-macro2/3-GE-Fc | Bernhard Lüscher | n/a | 1:1,000 | 1:50 | Mouse IgG | No | — |
IV | Anti-MAR | Michael Hottiger | n/a | 1:4,000 | 1:500 | Rabbit IgG | Yesa | Hopp et al (2021) |
V | Anti-PAR/MAR (E6F6A) | Cell Signaling Technology | #83732 | 1:50,000 | 1:1,000 | Rabbit IgG | Yesa | Lu et al (2019) |
VI | Pan-ADPr | Ivan Matic | 33,641 | 1:1,000 | 1:500 | Human | No | Bonfiglio et al (2020) |
VII | Anti-PAR | Trevigen | 4336-BPC-100 | 1:1,000 | 1:100 | Rabbit IgG | Yesa | — |
Stored at 4°C in TBST with 0.05% sodium azide.