Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Nov 10;222(1):e202202064. doi: 10.1083/jcb.202202064

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Wei et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S3. — The effect of the absence of Opy1 or overexpression of Opy1 on spore outgrowth and the localization of Opy1-2mNeonGreen and Rga6-tdTomato in its3-1 spores (Related to Fig. 6). (A) Maximum projection and Z-slice (Middle) images of WT, opy1Δ, and Opy1-overexpressing spores that were stained with Calcofluor-white. Note that stained spores were cultured in Calcofluor-white-free rich medium for 8 h before microscopic analysis. Pink arrowheads indicate bipolar growth of outgrowing spores. Scale bar, 10 μm. (B) The percentage of germinating WT, opy1Δ, and Opy1-overexpressing spores that underwent bipolar growth, as shown in A. Five independent experiments were carried out, and ≥ 25 spores were analyzed for each replicate. The top of the column and the error bar are the mean ± SD. One-way ANOVA with Tukey HSD post hoc test was used to calculate the P values. (C) Diagram showing the function of Its3 in synthesizing PI(4,5)P2. (D) Maximum projection images of Opy1-2mNeonGreen and Rga6-tdTomato-expressing WT and its3-1 spores cultured at the restricted temperature 37°C for the indicated time. Scale bar, 5 μm. (E) Maximum projection images of Opy1-2mNeonGreen and Rga6-tdTomato-expressing WT and its3-1 spores cultured at the permissive temperature 30°C for the indicative time. Note that both WT and its3-1 spores germinated at the permissive temperature. Scale bar, 5 μm.