Fig. 2.

Characterization of human umbilical cord blood-derived late EPCs. A Late EPCs expressed endothelial markers CD31, VE-cadherin, and VEGFR-2, but did not express hematopoietic marker CD133 by immunofluorescence staining. The cell nuclei were counterstained with Hoechst 33,342. A negative control was incubated with a secondary antibody only. Cells were visualized using an inverted fluorescence microscope (HPF, 400×). Scale bars = 60 μm. B) Temporalis muscles were subjected to IHC staining with Ku80 antibody (human-specific). Representative IHC staining images show Ku80-positive cells (red arrows, HPF 400×) 2 weeks after transplantation, indicating survival of transplanted EPCs in vivo. Scale bars = 100 and 25 μm (left and right, respectively)