Fig. 6.

Ca²⁺ oscillations and CaMKII mediate ACM C-MSC fibrotic differentiation. A Representative images of Collagen staining of ACM C-MSC cultured in GM low serum (2% FBS) and TGF-β1 (5 ng/mL) for 3 days supplemented or with CaMKII inhibitor or [Ca²⁺]_i modulators (as in Fig. 5), and HC C-MSC. Nuclei are stained with Hoechst 33,342. Magnification is 40X and the scale bar indicates 50 μm. B Quantification of images of ACM C-MSC marked with Collagen. The mean fluorescence of Collagen is shown for each condition relative to the values of basal level of ACM C-MSC in in GM low serum (BASE) (n = 3 biological replicates; one-way ANOVA test). Data information: mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001.