Fig. 5.

A. Immunohistochemical assays were used to detect distribution and location of PPARα in groups of mouse livers. (a) Regular chow; (b) MCD group; (c) MCD with Control Medium (CM) extract intervention group; (d) MCD with hUC-MSC exosomes(Ex) group. The random view to zoom in on the panel shown is on the right. Scale bar = 20 μm. B. Western blotting analysis of PPARα in liver tissues in groups of mice. Three samples were selected in each group (upper panel). Protein levels were quantified using ImageJ software and are represented by a histogram (fold change of PPARα/GAPDH, panel below). Full-length blots are presented in Additional file 1: Fig. S3. C. PKH-26 labeling of MSC-exo taken up by HepRG cells. Scale bar = 20 μm. D. PKH-26 labeling of MSC-exo taken up by Huh1-6 cells. Scale bar = 20 μm. E. Western blotting analysis of PPARα in the human liver cell line HepRG under the indicated treatment. Protein levels were quantified using ImageJ software and represented by a histogram (fold change of PPARα/Histone H3). **p < 0.01. Full-length blots are presented in Additional file 1: Fig. S4. F. Western blotting analysis of PPARα in the mouse hepatoma carcinoma cell line Huh1–6 under the indicated treatment (fold change of PPARα/Histone H3). Protein levels were quantified using ImageJ software and represented by a histogram. **p < 0.01. Full-length blots are presented in Additional file 1: Fig. S5