Fig. 2.

Astrocytic aSyn is modified at Tyrosine 39. a The specificity of the aSyn PTM antibodies against phosphorylation and nitration at Y39 were validated via antibody pre-blocking overnight followed by SB analysis. b The signal for astrocytic aSyn phosphorylated and nitrated at Y39 was lost with the antibody pre-blocking in the DLB1 cingulate cortex (layers V–VI). c The GFAP- and aSyn-positive astrocytes in the cingulate cortex of DLB1 were positive for aSyn nY39. aSyn = alpha-synuclein; DLB = dementia with Lewy bodies; GFAP = glial fibrillary acidic protein; PTM = post-translational modification; SB = slot blot