Fig. 3.

Astrocytic aSyn accumulations occur across LB disorders and may be truncated in the N- and C-termini. a CTR, PD, PDD, DLB, SNCA G51D mutation and SNCA duplication cingulate cortices were immunohistochemically stained using three aSyn antibodies, LASH-BL 34–45, LASH-BL 80–96 and BD SYN-1, and astrocytic accumulations (insets) were revealed across these LB disorders. b To further map the C-terminal truncation region of the astrocytic aSyn, the same cingulate cortex sections were stained using the C-terminal BL 4B12 antibody with an epitope 103–108 of aSyn. Neuronal inclusions were revealed, but the astrocytic aSyn accumulation was not detected, suggesting that the aSyn species associated with the astrocytes are truncated at residues 21–33 in the N-terminus, and at residues 100–102 in the C-terminus. Representative images in Fig. 3a, b taken from the cortical deep grey matter (layers V–VI) of CTR1, PD2, PDD2, DLB1, SNCA G51D3 and SNCA duplication. c A diagram to show the antibodies that are positive and negative for astrocytic aSyn, and their epitopes. The areas in stripes denote the potential truncation regions in the N- and C-termini. Schematic created with BioRender.com (agreement no: DJ23GJF70T). Scale bar for Fig. 3a is 20 µm for the main images and 40 µm for the insets. aSyn = alpha-synuclein; CTR = control; DLB = dementia with Lewy bodies; LB = Lewy body; PD = Parkinson’s disease; PDD = Parkinson’s disease with dementia