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. 2022 Nov 12;20:525. doi: 10.1186/s12967-022-03741-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Celastrol induced apoptosis of human NSCLC Cells. A After treatment with celastrol at the indicated concentration for 24 h before staining with Annexin V and propidium iodide (PI), the distribution of apoptotic cells was determined using flow cytometry. B The apoptotic characteristics in NSCLC cell nucleus presented by Hoechst Staining assay. After treatment with celastrol for 12 h before staining with Hoechst staining, the nucleus was observed and photographed (100×). Representative results of triple experiments. C Caspase 3 activity was evaluated using the Caspase Assay Kit, and absorbance was recorded at 405 nm. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001)