TABLE 2.
Mutant oligonucleotides and corresponding DtxR variants
| Oligonucleotidea | DtxR variant |
|---|---|
| Cation-binding ligands of site 1 | |
| 5′-ATGCGTAAAGCTCGCTTAGCT-3′ | H79A |
| 5′-CGCTTAGCTGCGCGCCTTCTT-3′ | E83A |
| 5′-AATAAAGTTGCCGATGAAGCC-3′ | H98A |
| Anion-binding ligands of site 1 | |
| 5′-CGTAAACATGCCTTAGCTGAG-3′ | R80A |
| 5′-GTCAGTCGGGCCCCCTTCGGA-3′ | S126A |
| 5′-CCCTTCGGAGCCCCAATTCCA-3′ | N130A |
| 5′-GAGCTGGAAGCAGAGGGAGTC-3′ | E20A |
| Metal binding site 2 | |
| 5′-CGATGAAGCCGCCCGCTGGGAAC-3′ | C102A |
| 5′-TGCCGCTGGGCACACGTTATG-3′ | E105A |
| 5′-CCGCTGGGAAGCCGTTATGAGTG-3′ | H106A |
| Multiple mutations | |
| 5′-TGCGTAAAGCTGCCTTAGCTGA-3′ | H79A/R80A |
| 5′-GCCTTAGCTGCGCGCCTTCTT-3′ | R80A/E83A |
a
Boldface print indicates those nucleotides that were changed relative to the wild-type dtxR sequence.