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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: J Med Chem. 2022 Oct 13;65(20):14015–14031. doi: 10.1021/acs.jmedchem.2c01215

Figure 3: Direct, dose-dependent activation of Sirt3 and Sirt5.

Figure 3:

(a) Sirt3 titrations with 11 and 31 result in dose-dependent Sirt3 activation. (b) MS-based deacetylation assay in absence and presence of 31 (100 μM), which confirms direct activation of Sirt3. Identical Sirt3 and substrate concentrations as in the coupled enzymatic assay were used. (c) Sirt5 titration with 30 results in dose-dependent Sirt5 activation. (d) MS-based desuccinylation assay in absence and presence of 30 (100 μM) confirming direct Sirt5 activation. Error bars: Standard errors from at least three independent experiments.