Table 1.
Primary antibodies and visualization methods used for cell type classification in human GBM and epilepsy tissue
| Target | Primary antibody | Company, catalogue number | Immunofluorescent visualization |
|---|---|---|---|
| Myeloid cells | Chicken anti-IBA1 | Synaptic Systems, 234-009 | IRDye® 800W secondary |
| Endothelia | Biotinylated Lectin from UEA-1 | Sigma Aldrich, L8262-2MG | AlexaFluor® 647 streptavidin |
| Pericytes | Goat anti-PDGFRβ | R&D Systems, Inc., AF385 | AlexaFluor® 594 secondary |
| GBM parenchyma/astrocytes | Mouse anti-GFAP | Sigma Aldrich, G3893 | AlexaFluor® 546 secondary |
| OATPs | Rabbit anti-OATP1A2 | Abcam, Ab221804 | AlexaFluor® 488 secondary |
| Rabbit anti-OATP2B1 | Thermo Fisher, PA5-42453 | AlexaFluor® 488 secondary | |
| Rabbit anti-OATP1C1 | Abcam, Ab234729 | AlexaFluor® 488 secondary | |
| Rabbit anti-OATP4A1 | Sigma Aldrich, HPA030669 | AlexaFluor® 488 secondary |