Figure 5.

The effect of RAB27A on colon cancer cell invasion. (A) and (B). RAB27A knockdown suppresses SW480 cell invasion. RAB27A knockdown and the negative control SW480 cells were seeded in the upper well of the Transwell chamber pre-coated with Matrigel for 48 h, and the invaded cells were stained with the crystal violet. (C) and (D). RAB27A overexpression facilitates RKO cell invasion. RAB27A overexpression and the negative control RKO cells were seeded in the upper well of the Transwell chamber pre-coated with Matrigel for 48 h, and the invaded cells were stained with the crystal violet. Cell invasion was determined by microscopy (200 ×). At least three fields in each group were observed, and the representative images were shown (scale bar = 100 µm). Invaded cell number in each field was calculated, shown as mean ± S.D. and analyzed by two-tailed t-test. The value of the vertical axes in this figure is invaded cell number per field of view. **P < 0.01. All the experiments were repeated three times independently and the representative results were shown.