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. 2022 Nov 12;8:454. doi: 10.1038/s41420-022-01243-7

Fig. 7. ITGB8 is a potential target gene regulated by LINC00472.

Fig. 7

A Analysis of RNA-seq overlap difference genes of HK-2 KO-NC/KO-PE and HK-2 KI-NC/KI-CMV. B The central sub network of the network which got by using PPI interaction analysis of differential genes produced by RNA-Seq in STRING database. RT-qPCR was used to detect the mRNA level of these network node molecules in HK-2 shNC and shLINC00472 cell line (C), and HK-2 KI-CTR and HK-2 KI-CMV cell line (D). E Western blot was used to detect the ITGB8 protein level in HK-2 and 769-P cells. The expression and distribution of ITGB8 in 769-P cell (F) and HK-2 cell (G) were detected by immunofluorescence (Left). Statistics of integrated density (Right). H RT-qPCR was used to detect the mRNA level of LINC00472 and ITGB8. I The correlation between LINC00472 and ITGB8. J Relationship between the expression of ITGB8 and the prognosis of RCCC patients. In C, D, F–H, data were shown as mean ± SD of three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).