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. 2022 Nov 12;13:6902. doi: 10.1038/s41467-022-34590-1

Fig. 1. Cell type taxonomy in the cynomolgus monkey primary visual cortex (V1).

Fig. 1

a Schematic illustration of single-cell transcriptome profiling of the macaque primary visual cortex (V1). The macaque V1 used for single-cell isolation and staining experiments is visualized in green. Drawings of macaque and single cells were created with BioRender.com. b Uniform Manifold Approximation and Projection (UMAP) visualization of the 133,454 cells of macaque V1. Exc excitatory neurons, Inh inhibitory neurons, Astro astrocytes, Oligo oligodendrocytes, OPC oligodendrocyte progenitor cells, Micro microglia, Vasc vascular cells. c Quantification of the percentage of different major cell types revealed by scRNA-seq datasets in macaque V1. d UMAP visualization colored by the enrichment dissection, cells collected from the tissue covering all layers are not shown. e The taxonomy of the 62 neuronal cell types (including 25 excitatory and 37 inhibitory types) and 5 non-neuronal cell types from the macaque V1. Branches are labeled with major cell populations.The dot-plot shows the mean expression level of selected genes. Cell types are labeled with layer distribution and their marker genes. Exc excitatory neurons. Inh inhibitory neurons. Astro astrocytes, Oligo oligodendrocytes, OPC oligodendrocyte progenitor cells, Vasc vascular cells. IT intratelencephalic, ET extratelencephalic, NP near-projecting, CT corticothalamic. f RNAscope mFISH confirms that HPCAL1 (green) is mainly expressed in a subset of SLC17A7 (red) glutamatergic cells in L2/3 and L6b. Blue, DAPI. Scale bar, 100 μm. g Quantifications of SLC17A7+HPCAL1+ cells across layers of macaque V1. Data are represented as mean ± s.d. and dots show the data points for individual macaques. The differences between groups were significant, as determined by one-way ANOVA. n = 3 macaques. h RNAscope mFISH for mRNA of SLC17A7 (red), HPCAL1 (green) and NXPH4 (light blue) are co-localized in L6b. Blue, DAPI. 6b, layer 6b; WM, white matter. Scale bar, 10 μm. i Counts of SLC17A7+HPCAL1+NXPH4+ cells. Data are represented as mean ± s.d. and dots represent biological replicates. n = 3 macaques. Source data are provided as a Source Data file.