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. 2022 Nov 12;12:19432. doi: 10.1038/s41598-022-22696-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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RNA sequencing of ARPE-19 cells for RPE and retinal markers. Wild type (WT), EMP2 overexpressing (OE), and EMP2 knock down (KD) ARPE-19 cells were queried for differential expression of classical RPE markers and retinal markers. A control cell line, MCF12A (immortalized breast cancer cell line) was analyzed in parallel, and results are presented as a heatmap generated in R studio using pheatmap, version 1.0.12 (https://CRAN.R-project.org/package=pheatmap.) High expression of RPE markers and low expression of retinal markers was observed in ARPE-19 cells, regardless of EMP2 expression. Both MCF12A and ARPE-19 cells showed low expression of most retinal markers (gray indicating zero transcripts identified). All samples were performed in triplicate.