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. 2022 Nov 12;12:19432. doi: 10.1038/s41598-022-22696-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EMP2 expression modulates HIF1α and VEGF protein expression. (A, B) Analysis of HIF1α or VHL protein expression in WT, shRNA control (shCTRL), OE, shRNA KD #1, and shRNA KD#2 ARPE-19 cells under normoxic or hypoxic (0.5% O2 for 2–4 h) conditions, respectively. Quantification of protein expression under hypoxic conditions was determined using the LI-Cor Odyssey FC machine with images visualized using Image Studio software (Ver. 5.2). Expression of HIF1α or VHL was normalized to beta actin, for a minimum of 3 independent replicates. Bar diagrams depict mean values and standard error of the mean. Statistical significance was established using Student’s t-test (unpaired, two-tailed) for HIF1a or a one-way ANOVA for pVHL. Original, uncropped blots are presented in Supplementary Fig. S3.