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. 2022 Nov 12;13:6881. doi: 10.1038/s41467-022-34660-4

Fig. 3. IL-33 causes thymic involution during schistosome infection.

Fig. 3

a Representative and quantified western blots of IL-33 (n = 3 mice). Infected+Veh versus uninfected or Infected+PZQ, P = 0.0029 or P = 0.0027. b–d Representative morphology and quantified weight and cellularity of the thymus (il33−/−+Sj, n = 6 mice; other groups, n = 8 mice; pool of two independent experiments), P < 0.0001. e Representative flow cytometry of thymocytes. f–i Mice were treated with anti-IL-33 at week 3 every other day until week 8 after infection. f–h Representative morphology and quantified weight and cellularity of thymus (n = 4 mice). Infected+Veh versus Uninfected+Veh or Infected+anti-IL-33, P < 0.0001 (weight or cells) or P = 0.0461 (weight), P = 0.0411 (cells). i Representative flow cytometry of thymocytes. j–m Mice were injected with IL-33 for six consecutive days. j–l Representative morphology and quantified weight and cellularity of the thymus (n = 8 mice, pool of two independent experiments). P < 0.0001 (weight), P = 0.0003 (cells). m Representative flow cytometry of thymocytes. n, o Representative morphology (n) and quantified weight and cellularity (o) of thymus (PBS, n = 7 mice; IL-33, n = 8 mice; pool of two independent experiments). Recipient WT thymus, P < 0.0001 (weight or cells); Donor il1rl1−/− thymus, P = 0.5391 (weight), P = 0.8775 (cells). p, q Representative morphology (p) and quantified weight and cellularity (q) of thymus (n = 4 mice). Infected versus Uninfected, P < 0.0001 (weight or cells) for recipient thymus; Infected+WT thymus versus Uninfected+ WT thymus, P < 0.0001 (weight or cells) for recipient thymus, P < 0.0001 (weight) or P = 0.0016 (cells) for donor thymus; Infected+il1rl1−/− thymus versus Uninfected+il1rl1−/− thymus, P < 0.0001 (weight or cells) for recipient thymus, P = 0.0071 (weight) or P = 0.0085 (cells) for donor thymus. All data are shown as the mean ± s.d; *P < 0.05, **P < 0.01, ***P < 0.001, NS, not significant. a, c, d, g, h, q One-way ANOVA with Tukey’s multiple comparisons; k, l, o Unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.