. 2022 Nov 12;12:141. doi: 10.1186/s13568-022-01480-0

Table 5.

Effect of Moringa oleifera leaf on digestibility, total gas, methane, PF and MBP along with different roughage and concentrate feed (80R:20C)

Parameters	0%	5%	10%	15%	20%	30%	40%	SEM	P value
Total gas (mL/gDM)	143.10 ± 3.78^c	151.08 ± 4.12^bc	154.26 ± 1.95^abc	163.68 ± 3.51^ab	163.95 ± 3.89^ab	166.15 ± 4.66^a	167.32 ± 1.87^a	2.307	0.01
IVTDMD (%)	60.31 ± 0.44^e	61.59 ± 0.75^de	63.90 ± 0.56^d	66.58 ± 1.54^c	70.52 ± 0.21^b	71.85 ± 0.68^ab	73.19 ± 0.37^a	1.089	< 0.001
IVTOMD (%)	62.86 ± 0.70^d	64.42 ± 0.07^d	67.73 ± 1.01^c	70.18 ± 0.21^b	72.24 ± 0.48^ab	74.24 ± 1.21^a	73.85 ± 0.67^a	0.962	< 0.001
CH4 (%)	34.23 ± 0.58^a	33.03 ± 0.10^ab	32.25 ± 0.21^b	30.53 ± 0.85^c	28.56 ± 0.57^d	27.94 ± 0.31^de	26.78 ± 0.17^e	0.601	< 0.001
CH4 (mL/100 mg)	8.89^a ± 0.50^a	8.61 ± 0.50^a	8.40 ± 0.12^a	7.90 ± 0.50^ab	7.05 ± 0.31^bc	6.86 ± 0.18^bc	6.56 ± 0.11^c	0.223	0.001
Acetate (mM)	47.92^d ± 1.24^d	51.70 ± 0.20^cd	55.76 ± 1.46^bc	57.46 ± 1.98^ab	57.23 ± 1.51^ab	61.90 ± 2.89^a	62.68 ± 1.54^a	1.217	< 0.002
Propionate (mM)	16.11 ± 0.77^c	16.80 ± 1.15^bc	18.30 ± 0.33^ab	18.79 ± 0.55^ab	19.37 ± 0.39^a	19.57 ± 0.87^a	20.19 ± 0.07^a	0.377	0.01
Butyrate (mM)	9.71 ± 0.43	9.58 ± 0.98	11.12 ± 0.07	10.31 ± 0.50	9.82 ± 0.76	8.97 ± 1.12	9.24 ± 0.48	0.267	0.45
AP ratio	2.99 ± 0.20	3.10 ± 0.20	3.05 ± 0.08	3.06 ± 0.04	2.95 ± 0.03	3.16 ± 0.09	3.10 ± 0.07	0.041	0.90
MBP (mg)	42.65 ± 1.25^d	45.38 ± 0.95^cd	47.63 ± 3.2^bcd	51.34 ± 0.20^abc	53.28 ± 2.59^ab	54.43 ± 2.48^a	56.82 ± 1.08^a	1.234	0.001
PF	2.90 ± 0.17	2.84 ± 0.20	2.92 ± 0.06	2.84 ± 0.17	2.91 ± 0.13	2.98 ± 0.09	2.81 ± 0.07	0.04	0.910
Ammonia N (mg/dl)	13.07 ± 0.46	12.60 ± 0.80	12.60 ± 0.80	12.13 ± 1.68	13.67 ± 0.54	11.90 ± 1.212	11.83 ± 0.46	0.331	0.814

Means with different superscripts a, b, c, d and e in the same row differ significantly (P < 0.05)