Table 1.
The differential expressed proteins in L. tropica metacyclic and amastigote-like stages
| FC AT/MT | Uniprot IDs | Protein name | Gene name | Peptides |
|---|---|---|---|---|
| Up-regulated proteins list during metacyclic conversion into amastigote-like form of L. tropica | ||||
| 12.91 | E9ACW0 | Putative heat shock protein DNAJ | LMJF_27_2400 | DIVHELPVPLEAFYCGK |
| 7.53 | E9ADS8 | Putative lipophosphoglycan biosynthetic protein | LMJF_29_0760 (LPG3) | MLDILVNSLYTNR |
| 2.05 | E9AF45 | Kinetoplastid membrane protein 11 | LMJF_35_2210 (KMPII-1) | FAELLEQQK, LDRLDEEFNRK, EHSEHFK |
| 3.83 | Q4Q1M0 | Chaperonin HSP60, mitochondrial | LMJF_36_2030 | IQSIHSLLPALNHVVR, TGVTIVR,KIQSIHSLLPALNHVVR, AVAAVATTLGPK |
| 7.28 | Q4Q1R4 | Putative universal minicircle sequence binding protein | LMJF_36_1610 (UMSBP1) | CGEAGHMSR |
| 41.32 | Q4Q1Y2 | Putative 40S ribosomal protein S18 | LMJF_36_0940 | SLTLIPDHFQHIVR,FKIPDWFLNR,TEHLSSSMVDTRAGTLTAEELEKIAEIIADPAK, HAYGLR |
| 13.02 | Q4Q3V3 | Succinyl-CoA:3-ketoacid-coenzyme A transferase | LMJF_33_2340 | SGNLVFR, QTGGQIIR, GPGGAMDLVASGSR |
| 21.18 | Q4Q5P6 | Putative 26S proteasome regulatory subunit | LMJF_32_0390 | VAGLLLGR, HTNDEAIATFLAAIAR |
| 22.5 | Q4Q822 | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex | LMJF_28_2420 | GLVVPVIR, LGLMSPFVK, NLIEDPAR |
| 6.68 | Q4Q931 | Putative 40S ribosomal protein S33 | LMJF_28_2420 (S33-1) | ENDMLSLMETER, GNVTQVR, LMAEAGSPDYNR |
| 78.77 | Q4Q9X6 | ATP synthase subunit beta | LMJF_25_1170 | IFNVLGDAIDQR, VAQSALTMAEYFR, GHGGFSVFAGVGER,FTQANSEVSALLGR, TVIIMELINNVAK |
| 3.0 | Q4QAB9 | Uncharacterized protein | LMJF_24_2110 | ALENPVNLDK, MEFVIDR, NEAAFQDVGIEYYR |
| 20.39 | Q4QD34 | Phosphoglycerate kinase | LMJF_20_0100 (PGKC) | SALPTIQK, EGGSCILMSHLGRPK, VLGAGYAGYLMEK |
| 2.73 | Q4QDF0 | Glycosomal malate dehydrogenase | LMJF_19_0710 (Gmdh) | RDPALAELAK, GSATLSMAEAGAR,VQVAGTEVVK, DPALAELAK, LLGVSLLDGLR |
| 5.04 | Q4QGX4 | Putative pretranslocation protein,alpha subunit | LMJF_11_1050 | QANWLMSLKPMLAVLPEIEKPR |
| 6.15 | Q4QJF1 | ATPase alpha subunit | LMJF_05_0500 | VDAGAPNIVSR, SPVNYNLLTGFK, FVALFNQK, VVNPLGHEVPVGL, AVDTMIPIGR |
| 2.0 | Q9U0V9 | Possible 3-ketoacyl-CoA thiolase | LMJF_23_0690 (L7836.03) | LDDFTFPCLFAK, KHPDFGK |
| 14.28 | E9ACG7 | Putative delta-1-pyrroline-5-carboxylate dehydrogenase | LMJF_03_0200 | YGLTGAVFSR, GAFEFQGQK, CTGAVVGQQPFGGSR, GYFVEPTIIETK |
| 9.32 | E9AFE7 | Putative cystathione gamma lyase | LMJF_35_3230 | NNLHGGMLWFEVK, VGITDGFVR, NNLHGGMLWFEVK, NNLHGGMLWFEVK |
| Down-regulated proteins list during metacyclic conversion into amastigote-like form of L. tropica | ||||
| 8.28 | O62591 | Probable eukaryotic initiation factor 4A | LMJF_01_0770 | HNLIQGLVLSPTR, VLVTTDLVAR,HNLIQGLVLSPTR, ESLTLEGIK |
| 28.72 | Q4FX73 | 40S ribosomal protein S3a | LMJF_35_0400 | NVLSDALVR, FTVQEVQGR, EWYDVVAPANFEK |
| 4.81 | Q4QEB3 | GMP reductase | LMJF_17_0725 (GMPR) | IGVGPGSICITR, LIVGAAIGVK, GPLAPILK |
| 2.47 | Q4QG98 | 60S ribosomal protein L18 | LMJF_13_0560 (RPL18-A) | GVDLTGISK, AAPIAVVVGDVLDDVR |
| 2.84 | E9AD27 | Putative calpain-like cysteine peptidase | LMJF_27_0500 | SIFLPLNTFLK, AELQRAVLKAQNAK, NATAIQDLEEALNDR |
| 26.07 | E9AD53 | Putative small GTP-binding protein Rab1 | LMJF_27_0760 | LLLIGDSGVGK, DFADSLGIPFLETSAK |
| 3.47 | E9ADF9 | Putative glycosomal phosphoenolpyruvate carboxykinase | LMJF_27_1810 | VAYPLEHIPGALTHAVAGHPNNVIFLTNDAFGVMPPVAR, NLTAPELVQWALK, GALCVL SYAK, KGDVTVFFGLSGTGK, GVFNIEGGCYAK |
| 17.39 | E9ADX3 | Tryparedoxin | LMJF_29_1150 (TXN2) | MPWLALPFEDRK |
| 3.17 | E9AE57 | Putative fumarate hydratase | LMJF_29_1960 | HGGFYLGSIGGPAAILAK, YFAHQAR, YVEEVEVFGR |
| 2.12 | E9AEB3 | ATP-dependent 6-phosphofructokinase | pfk | TAIELSR, TIDNDLAFSHR, FGGTILGSSR,HLHFNPSETSIGIVTCGGICPGLNDVIR, EMVDTLVR |
| 67.7 | E9AEL4 | Putative ATP-dependent DEAD-box RNA helicase | LMJF_35_0370 | TASFVIPVLEK, VHILVATPGR, GFEKPSPVQEEAIPVALQGK, HIPGLEVMVTTGGTTLR, ELALQTAQVTK, NVNFEEYALR |
| 4.36 | E9AEU1 | Putative NADH-dependent fumarate reductase | LMJF_35_1180 | LGGNSLLECVVFGK, AATILQK, ATSGINAWGTR, LALIGGGTGVAPMLQIVR, LIGCPEANVMATLK |
| 6.1 | E9AF23 | 40S ribosomal protein S6 | LMJF_35_2010 | LFNLSR, GAIGFNTFR, RGAIGFNTFR,RVQLQDYR, VGDQPIEGVTDTTAPR |
| 2.83 | E9AFK3 | Putative 60S ribosomal protein L23 | LMJF_35_3790 | VLNAVIIR, ISTHAPAIV, NLYVISVK |
| 36.1 | Q4Q090 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase | PGAM | VALQGASLVDDALK, MFVTMDR, SAEITEAAIEALK, VALQGASLVDDALK |
| 2.64 | Q4Q124 | Adenosylhomocysteinase | LMJF_36_3910 | AGVFFLPK, VAALHLAHVGAK, DISLAEWGR, EHVEIKPQVDR, VKDISLAEWGR, FDNLYGCR |
| 7.9 | Q4Q1D2 | 40S ribosomal protein S24 | S24E-2 | TTGFGLIYDDLASLK |
| 2.8 | Q4Q1F5 | Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex | LTITPIPMPALSPTMEK, WFQHFHDAMENPLSLLL | |
| 2.49 | Q4Q1X7 | Putative 40S ribosomal protein S10 | LMJF_36_0980 | FFFTEGVIACK |
| 7.71 | Q4Q230 | Uncharacterized protein | LMJF_36_0480 | KSPIMSK, LMDQSLPVYDDVVTGVGR |
| 2.59 | Q4Q2H7 | Putative vacuolar ATP synthase catalytic subunit A | LMJF_34_3670 | ITWNYIR, NIVTFYEEAQR, TCLVANTSNMPVAAR, EEELQEIVQLVGK |
| 7.61 | Q4Q3U8 | Putative heat shock protein | LMJF_33_2390 | YNLHFNPQHPLIR, GLLPDWLR, EELTANLGTIAGSGSK |
| 2.74 | Q4Q4U1 | Dihydrolipoyl dehydrogenase | GCVL-2 | ALTGGVEYLFK, AAQLGLK, AVGTEDGFVK |
| 3.42 | Q4Q5P0 | 40S ribosomal protein S2 | LMJF_32_0450 | GTGIVAAPVPK, THGNLIMATFYALR |
| 8.42 | Q4Q6E1 | Putative vacuolar-type proton translocating pyrophosphatase 1 | LMJF_31_1220 | QFQDPEVAEGR |
| 3.81 | Q4Q9H4 | Putative 60S ribosomal protein L7 | KILQLLR, AVEPYIAYGYPSLATVR | |
| 35.55 | Q4Q9M4 | Succinate–CoA ligase [ADP-forming] subunit alpha, mitochondrial | LMJF_25_2130 | VIVQGMTGK, VVGGVSPK, VIVQGMTGK, AGTFHTK |
| 3.93 | Q4Q9R2 | Polyprenol reductase | EnCR | DLGPQIGYR, ELESMFVHK, FSHPTMPMR |
| 2.22 | Q4Q9V1 | GTP-binding nuclear protein | LMJF_25_1420 | LILVGDGGTGK, SNYNFEKPFVWLAK, VCDNIPIVLVGNK |
| 5.74 | Q4Q9Y0 | Putative cytochrome c oxidase VII | LMJF_25_1130 | IPNPFAYSFK, VWAPATTLAEYR |
| 5.22 | Q4QAG8 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial | LMJF_24_1630 | AITMEILAGR, LGANSLLDIVVFGK, GEGGYLVNSEGER, SPVWNSNLIEALELR |
| 18.71 | Q4QAX6 | Putative 60S ribosomal protein L17 | LMJF_24_0040 | HVQVDQAAR, SVVAMMSLLK |
| 2.0 | Q4QEI9 | Elongation factor 1-alpha | LMJF_17_0080 | IGGIGTVPVGR, GITIDIALWK, FESPKSVFTIIDAPGHR, SVFTIIDAPGHR, EHALLAFTLGVK, STATGHLIYK |
| 7.48 | Q4QEM2 | Paraflagellar rod protein 2C | LMJF_16_1425 | AQLLEHLVELVADKFR, TLGQLVYK |
| 14.07 | Q4QEX4 | Putative 60S ribosomal protein L21 | LMJF_16_0460 | GVGVIINKPVR, TGIVWNVTPR, VGDYVDVVADSAVR |
| 33.52 | Q4QF62 | 60S acidic ribosomal protein P2 | LMJF_15_1203 | AVHIDVDQATLAFVMESVTGR, ASPSQADVEAICK |
| 23.38 | Q4QF80 | Tryparedoxin peroxidase | TRYP1 | GLFIIDPHGMLR |
| 6.15 | Q4QFF2 | Putative ribonucleoprotein p18, mitochondrial | LMJF_15_0280 | FCAMMDLMEEMQHR, FCAMMDLMEEMQHR, NCPPDLETYNATLQK |
| 19.25 | Q4QFG2 | Putative 60S ribosomal protein L13a | LMJF_15_0200 | APSDVFVR, HRPEIIVIDLK, HRPEIIVIDLKDHVLGR, CEQLNIAGTEIR |
| 2.34 | Q4QFL8 | Enolase | ENOL | HIDEPLPILMEAIEK, LPVPCFNVINGGK |
| 181.11 | Q4QFP8 | Putative small myristoylated protein-3 | SMP-3 | ISFEANPIAK, DNGNGLLFR |
| 5.92 | Q4QG31 | 40S ribosomal protein S4 | RS4 | LRECLPLLVIIR, AVIVTGGANR, ECLPLLVIIR,DLNNLQVTVPK, MNVIQER,DASGAEFATR |
| 3.49 | Q4QGA9 | Uncharacterized protein | LMJF_13_0450 | SPEFDAIYEQQQK |
| 2.77 | Q4QGC5 | Tubulin alpha chain | LMJF_13_0280 | LIGQVVSSLTASLR, IHFVLTSYAPVVSAEK, EIVDLALDR, QLFNPEQLVSGK, AVCMIANSTAIAEVFAR |
| 2.59 | Q4QGN9 | Glucose-6-phosphate isomerase | PGI | AVLHVALR, HFVALSTNTEK, PSNSILVNALTPR, QVNLEETIFIIASK |
| 22.93 | Q4QIP1 | Putative 60S ribosomal protein L7a | LMJF_07_0500 | APLAVVTGLQEVTR, WPTFVTMQR,TATCVALTDVNAEDEATLK |
The list of differential expression proteins based on fold change > 2 and p-value < 0.05 during developmentally process from metacyclic promastigotes to amastigotes like in Iranian L. tropica isolates
FC fold change, AT amastigote of L. tropica, MT metacyclic of L. tropica