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. 2022 Oct 3;11(1):31–40. doi: 10.22088/IJMCM.BUMS.11.1.31

Table1.

List of specific primers used for quantitative real time RT-PCR

Amplicon size (bp) Tm (°C) Sequence (5’>3’) Gene Bank
accession no.
Genes
259 58 ACGAAGACATCCCACCAATC NM_001100.4 hCol1a1-F
ATGGTACCTGAGGCCGTTC NM_001100.4 hCol1a1-R
70 58 AGGATGGTTGCACGAAACAC NM_000090.4 hCOL3A1-F
ACAGCCTTGCGTGTTCGATA NM_000090.4 hCOL3A1-R
79 59.5 CCCTGGGGAACACTACATTTTG NM_001244438.2 ARG1-F
GCCAATTCCTAGTCTGTCCACTT NM_001244438.2 ARG1-R
202 58 CAGCAACAATTCCTGGCGAT NM_000660.7 TGFB1-F
GGTAGTGAACCCGTTGATGT NM_000660.7 TGFB1-R
248 60 ATTTTTCCCTCGACACCGAT NM_Z35402.1 E. Cadherin-F
TCCCAGGCGTAGACCAAGA NM_Z35402.1 E. Cadherin-R
172 58 GAAGGTGAAGGTCGGAGT NM_001357943.2 GAPDH-F
GAAGATGGTGATGGGATTTC NM_001357943.2 GAPDH-R