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L. monocytogenes actA/plcB is not expressed in primary host cell vacuoles. PtK2 cells were infected with NF-L404 (Δhly actA-gfp; A and B) or NF-L411 (NF-L404 plus plasmid-encoded hly; C and D). Infected cells were fixed and stained (A and C) or examined for fluorescence (B and D) at 8 h postinfection. At least 50 microscopic fields were examined per experiment; one of three experiments with similar results is shown.
