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. 2022 Oct 31;14(21):5366. doi: 10.3390/cancers14215366

Figure 2.

Figure 2

(A) Cas9/sgRNA delivery using AuNPs and gene editing. (a) Cas9 protein engineered with ArgNPs for intracellular delivery of Cas9-protein/RNP through membrane fusion. Illustration of Cas9 transporting an E-tag at the N-terminus and a nuclear localization signal at the C-terminus (NLS). (b) Chemical structure of ArgNPs. (c) Nano assembly of Cas9En-RNP and ArgNPs. (d) A membrane fusion method for delivering Cas9En (reprinted with permission from [35]). (B) Conjugation of Cas9 nuclease and carrier NPs makes CRISPR component packing and transport more efficient. Cas9En self-assembled into massive nano assemblies with arginine-functionalized-AuNPs (reprinted with permission from [35]).