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Determination of multimeric forms of PA-LF complex. Purified PA63 (468 ng) was incubated with various amounts of LF for 30 min at room temperature. Samples were separated on 4 to 15% Phast gradient gels. Lanes 1 to 8, increasing amounts of LF (0, 47, 94, 187, 374, 515, 562, and 936 ng, respectively). The mass ratio of LF to PA is indicated above each lane. The results are representative of the three analyses performed.
