FIG. 3.

Potency of PA oligomer bound to various ratios of LF. Aliquots of purified PA63 at 10 μg/ml were incubated with various amounts of LF for 30 min at room temperature. The resulting PA63-LF complexes were serially diluted and added to the RAW264.7 cells and incubated for 2.5 h at 37°C in a CO₂ incubator. The labels on the lines refer to the molar ratios of PA63 to LF during the preincubation. For the curve “PA-LF,” LF was added at 0.5 μg/ml to all wells and the indicated concentration of PA63 was added separately. Cell lysis was determined by MTT assay. The results are representative of the three analyses performed.