Figure 3.

Isoquercitrin significantly decreased influenza A viral protein expression. PR8-GFP IAV at 10 MOI and 0, 2.5 µM, or 25 µM IQC were mixed and cotreated to the RAW 264.7 cells, as described in Figure 2. After fixing with paraformaldehyde at 24 h post-infection, the cells were immuno-stained with specific antibodies against M2, NP, PB1, PA, HA and NA (Red). The nuclei were stained in blue color with Hoechst 33342. The co-localization of viral proteins and nuclei was observed using a fluorescent microscope.