Figure 1.

Phylogenetic context for the five plastome inversions (numbered red hash marks) in the lineage leading to Lobelia fervens and L. erinus (redrawn from [4]). The temporal order of the accD gene loss and duplicative transposition of rpl23 in the common ancestral lineage of the Campanulaceae, Cyphiaceae, and Lobeliaceae cannot be determined, but insertion of the ancestral ORF200 (blue hash marks indicate the insertion of foreign ORFs) is inferred to have occurred subsequently. Lobelia thermalis only has Inversion 1 in the LSC (the two subsequent inversions are in the IR), L. jasionoides (and many other species) only have Inversions 1 and 2, and L. laxa has four of the five inversions.