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. 2022 Oct 22;11(21):3338. doi: 10.3390/cells11213338

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence staining of SMAD 2/3 (antibody conjugated with AF-546, green), nuclei of the cells stained with Hoechst 33342 (blue), in the following conditions: LX2, activated LX2 (induced with TGF-β1), activated LX2 + ADSC (activated LX2 cocultured with ADSCs), activated LX2 + HGF (activated LX2 treated with HGF), activated LX2 + ADSC + HGF (activated LX2 cocultured with ADSC, treated with HGF); scale bar: 50 µm.