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. 2022 Oct 27;23(21):13009. doi: 10.3390/ijms232113009

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometric analysis of neuropeptide Y receptors on untreated control M0 and polarized M(IFN-γ/LPS) and M(IL-10) macrophages. Cells were stained with primary antibodies anti-NPY Receptor Type 1 (NPY R Y1), type 2 (NPY R Y2), and type 5 (NPY R Y5), further detected by secondary antibodies conjugated to Alexa Fluor-488 and analyzed by flow cytometer. Histograms show the percentages of positive cells (%) and the mean fluorescence intensity (MFI). Results are expressed as mean value ± SD of 4 independent experiments. Significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis; *: p < 0.05, ***: p < 0.001.