Figure 4.
Flow cytometric analysis of endocytosis of FITC-dextran in M0 and polarized M(IFN-γ/LPS) and M(IL-10) macrophages stimulated with different concentrations (10−8–10−10 M) of NPY in the presence or absence of microbial LPS. After 24 h, treated and untreated cells (1 × 106 cells/mL) were incubated with FITC-dextran (1 mg/mL) for 45 min. The cellular uptake was then analyzed by flow cytometry. Results are expressed as mean fluorescence intensity (MFI) (mean ± SD; N = 4). Significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis; *: p < 0.05, **: p < 0.01.