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. 2022 Oct 27;23(21):13009. doi: 10.3390/ijms232113009

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determination of intracellular lipid droplet levels in M0 macrophages stimulated with 7-keto-cholesterol (7KC) in the presence or absence of NPY. M0 macrophages pretreated with 10⁻⁸ M NPY for 1 h were stimulated with 7KC for 24 h and then the median fluorescence intensity (MFI) of the intracellular LipidSpot™ dye was analyzed. (a) Representative flow cytometric analysis of intracellular lipid droplet levels MFI values is indicated. Histogram marker B designates the % of positive staining cells. (b) Flow cytometric analysis of lipid droplets (LipidSpot^TM MFI) expressed as mean ± SD from 4 independent experiments. Significance was determined by one-way ANOVA followed by Tukey’s post hoc analysis; **: p < 0.01.